Journal: BMC Genomics

Article Title: Real-time analysis of nanopore-based metagenomic sequencing from infected orthopaedic devices

doi: 10.1186/s12864-018-5094-y

Figure Lengend Snippet: Species detected after read classification and reference genome alignment in CRuMPIT

Article Snippet: Cycling conditions were an initial denaturation at 95 °C for 1 min, followed by 40 cycles of 95 °C denaturation for 15 s and 60 °C extension for 30 s. Genomic DNA, extracted from cultures of A. haemolyticum (Type Strain NCTC 8452) and F. nucleatum subspecies vincentii (Type Strain ATCC 49256), was diluted to 100,000 genome copies per μl then serially diluted to 10 genome copies per μl and used to create copy number standard curves for both species.

Techniques: Sonication

Journal: BMC Genomics

Article Title: Real-time analysis of nanopore-based metagenomic sequencing from infected orthopaedic devices

doi: 10.1186/s12864-018-5094-y

Figure Lengend Snippet: qPCR results

Article Snippet: Cycling conditions were an initial denaturation at 95 °C for 1 min, followed by 40 cycles of 95 °C denaturation for 15 s and 60 °C extension for 30 s. Genomic DNA, extracted from cultures of A. haemolyticum (Type Strain NCTC 8452) and F. nucleatum subspecies vincentii (Type Strain ATCC 49256), was diluted to 100,000 genome copies per μl then serially diluted to 10 genome copies per μl and used to create copy number standard curves for both species.

Techniques: